QA-QC Protocols and Spectra

ESI tuning and calibration of the Agilent QTOF 6540 is achieved using the Agilent test mix (table). This tune mix is always present in bottle B under the ion source.

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The system is put ?in the ON state: In the Context pull down, select Tune.

Select calibrant bottle B for the calibrant mixture to flow into the ion source. The flow stream from LC should go to waste. Select polarity. Make sure all the calibrant masses are selected.

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Verify that the resolution of mass 922.0097 (Positive ion mode) mass 1033.9881 (negative ion mode) is about 40,000 (FWHM).

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The deviation across the mass range should be under 1 ppm.

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